Project

Part:BBa_K2036013:Design

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-19)


RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1168


Design Notes

When CII is expressed it can further activate GFP and forming a Switching Lag-times. And in HUST-China 2016 designed circuit,this composite is constructed to test CII and pRE interaction.


Source

CII and pRE are a part of bactriaphage lambda operon.

References